Protein_Domain
Holin, T4

Part:BBa_K112003:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)


T4 holin, without start or stop codon, berkeley standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

References